Contractile Force & Electrophysiology Phenotyping
Functional acceptance testing of engineered tissue — twitch and tetanic force, fatigue, damage, and electrophysiological activity.
| Category | QC, Characterization & Acceptance Testing |
|---|---|
| Unit | One phenotyping campaign across a defined number of tissues and conditions |
| Lead time | 3-5 weeks for a typical phenotyping study per published vendor phase duration; quote-dependent |
| Pricing | By quotation |
| Provenance | Custom RFQ |
| Data current | 2026-09-01 |
Description
Functional rather than structural QC: does the tissue actually do work. Curi Bio's Curi Engine 3D phenotyping phase explicitly engineers customer cells into 3D tissues and runs skeletal muscle function assays including tetanic force, fatigue and damage protocols, with a typical study duration of 3-5 weeks. The published Mantarray validation data gives concrete performance envelopes for what these measurements return: iPSC-derived engineered muscle tissue average twitch force rising from about 385 uN at day 7 to about 1,156 uN by day 10, average tetanic force about 1,660 uN, and primary human myoblast constructs around 306 uN twitch at day 7 and continuing to rise through day 14. Those numbers are what make a force-based acceptance criterion writable rather than aspirational. This is the offering that converts our contractile tissue work from a hopeful order into a specification with a pass/fail test, and it should be contracted as a separate line item so that the builder is not the sole judge of whether the build succeeded. Quote-only.
Why this is hard to buy today
Force phenotyping is currently bundled inside a drug-discovery service contract rather than sold as standalone acceptance testing, so there is no way to independently verify that a tissue you commissioned meets its spec.
Price evidence
No public price. Published performance reference points from the Mantarray validation paper: iPSC-derived engineered muscle tissue average twitch force 385-1,156 uN and average tetanic force about 1,660 uN; primary human myoblast constructs about 306 uN twitch at day 7.
These are supplier list prices we located and recorded, not a quotation from Wetware World. Your price depends on quantity, specification and freight, and is only binding once issued in writing.
What you must specify
A quote cannot be produced without these. Anything you leave open, a supplier will assume — usually expensively.
| # | Parameter |
|---|---|
| 01 | Measurement type (twitch force, tetanic force, fatigue protocol, damage protocol, calcium imaging, MEA electrophysiology) |
| 02 | Acceptance threshold in physical units and the exact protocol used to measure it |
| 03 | Number of tissues and technical replicates |
| 04 | Stimulation parameters during measurement |
| 05 | Timepoints across maturation |
| 06 | Whether measurement is destructive and what happens to units afterwards |
| 07 | Raw waveform data delivery requirement |
| 08 | Independence requirement (tested by the builder vs by a third party) |
Quality documentation
Typically available with this item. What actually ships is confirmed on the quotation, per supplier.
- Per-unit force traces against the acceptance criterion
- Instrument calibration record
- Measurement protocol documentation
- Statistical summary across the batch
Candidate suppliers
Organisations we have identified as capable. Listing does not imply a partnership, an endorsement, or that they have agreed to quote.
| Organisation | Basis for inclusion |
|---|---|
| Curi Bio (Curi Engine services) | Phased fee-for-service: CRISPR iPSC editing 8-16 wk, iPSC expansion/banking 4-6 wk, directed differentiation 4-8 wk, 3D phenotyping study typically 3-5 wk, screening 4-12 wk. Deliverable is a report; whether they will sell the physical tissue is unconfirmed. |
| Curi Bio | Mantarray 24-well tissue casting plate kit, Stimulation Kit lid, MantaReady media, myoblasts and iPSC lines. 'Request a Quote' on every SKU — no public price. Claims >95% tissue formation success rate. |
| eNUVIO (contract research) | Service list explicitly includes '3D MUSCLE CULTURES // NEUROMUSCULAR JUNCTION MODELS (2D-3D)' and 'NEURAL SPHEROIDS'. States 'we do not offer off-the-shelf assays but rather develop customized assays'. |
Sources
doc WW-QC-30903 · rev 2026-09-01 · research use only · prices indicative and not binding until quoted in writing